APA
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Zuani, M. D., Xue, H., Park, J. S., Dentro, S., Seferbekova, Z., Tessier, J., … Cvejic, A. (2024). Single-cell and spatial transcriptomics analysis of non-small cell lung cancer. Nature Communications.
Chicago/Turabian
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Zuani, Marco De, Haoliang Xue, Jun Sung Park, S. Dentro, Z. Seferbekova, Julien Tessier, Sandra Curras-Alonso, et al. “Single-Cell and Spatial Transcriptomics Analysis of Non-Small Cell Lung Cancer.” Nature Communications (2024).
MLA
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Zuani, Marco De, et al. “Single-Cell and Spatial Transcriptomics Analysis of Non-Small Cell Lung Cancer.” Nature Communications, 2024.
BibTeX Click to copy
@article{marco2024a,
title = {Single-cell and spatial transcriptomics analysis of non-small cell lung cancer},
year = {2024},
journal = {Nature Communications},
author = {Zuani, Marco De and Xue, Haoliang and Park, Jun Sung and Dentro, S. and Seferbekova, Z. and Tessier, Julien and Curras-Alonso, Sandra and Hadjipanayis, Angela and Athanasiadis, E. and Gerstung, M. and Bayraktar, O. and Cvejic, A.}
}
Lung cancer is the second most frequently diagnosed cancer and the leading cause of cancer-related mortality worldwide. Tumour ecosystems feature diverse immune cell types. Myeloid cells, in particular, are prevalent and have a well-established role in promoting the disease. In our study, we profile approximately 900,000 cells from 25 treatment-naive patients with adenocarcinoma and squamous-cell carcinoma by single-cell and spatial transcriptomics. We note an inverse relationship between anti-inflammatory macrophages and NK cells/T cells, and with reduced NK cell cytotoxicity within the tumour. While we observe a similar cell type composition in both adenocarcinoma and squamous-cell carcinoma, we detect significant differences in the co-expression of various immune checkpoint inhibitors. Moreover, we reveal evidence of a transcriptional “reprogramming” of macrophages in tumours, shifting them towards cholesterol export and adopting a foetal-like transcriptional signature which promotes iron efflux. Our multi-omic resource offers a high-resolution molecular map of tumour-associated macrophages, enhancing our understanding of their role within the tumour microenvironment.